Understanding the importance of the force associated to the domain movements of BiP during protein translocation in the endoplasmic reticulum. To determine the mechano-chemistry of protein transport, we will use  optical tweezers (OT) to characterize the dynamics and force generating step(s) during the polypeptide translocation mediated by Bip and SecA at the single molecule level.

Team

• Christian A.M. Wilson Moya (Uchile, Biochemistry)
• Diego Quiroga (Uchile, Biochemistry)
• Francesca Burgos (Uchile, Biochemistry)
• Hilda Alfaro (Uchile, Biochemistry)
• Miguel Lagos (Uchile, Biochemistry)

Article

Single-molecule measurements of the effect of force on Thy-1/αvβ3-integrin interaction using nonpurified proteins.

 

Book chapter

Mechanical Properties of Chaperone BiP, the Master Regulator of the Endoplasmic Reticulum

Review